The goal of the course is to introduce current and newly emerging technologies and approaches in protein and proteome analysis with regard to their application in biology, biotechnology and medicine.Format: Introduction by instructor followed by discussions (mornings) stimulated by reading assignments and exercises (afternoons).

The course will introduce students to a selected set of laboratory techniques that are foundational to modern biological research.

This course will focus on molecular mechanisms and concepts underlying cellular biochemistry, providing advanced insights into the structural and functional details of individual cell components, and the complex regulation of their interactions. Particular emphasis will be on the spatial and temporal integration of different molecules and signaling pathways into global cellular processes.

This introductory Laboratory course introduces the student to the entire range of classical and modern molecular biosciences. In the second year (Praktikum GL Bio II) the students will perform three praktikum days in:- Plant Biology- Animal Models- Genomics- Molecular Systems Biology(total of 12 experiments)Each experiment takes one full day.

Protein Analysis by Mass SpectrometryThe following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.

The course will teach the basis and typical applications of methods for the analysis of nucleic acid sequences, mass spectrometric analysis of proteins and proteomes and advanced light and fluorescent imaging methods.

Introduction to experimental and computational methods of systems biology. By using baker’s yeast as a thread through the series, we focus on global methods for analysis of and interference with biological functions. Illustrative applications to other organisms will highlight medical and biotechnological aspects.