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551-0352-00L 6 Credits BSC D-BIOL
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Introduction to Mass Spectrometry-Based Proteomics

Lecturers & Examiners: Prof. Dr. Paola Picotti, Dr. Ludovic Gillet, Dr. Valentina Cappelletti
Number of participants limited to 12. The enrolment is done by the D-BIOL study administration.
VVZ CR n/a

Last Updated: 2026-02-05 16:30:53

Abstract

Protein Analysis by Mass SpectrometryThe following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.

Objective

How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins

Resources

Learning Materials (Links)

General Information

Language
English
Levels
BSC
Frequency
Yearly recurring

Examination

Type
graded semester performance
Students are obliged to be present throughout the block course.Cancellation: If you have to deregister from a course that has been assigned to you (just emergency reasons), please notify in written the course coordinator at least four weeks before the course starts, for courses in the 1st quarter, a cancellation period of one week applies. The study secretariat D-BIOL must also be informed (email CC [email protected]) so that the enrollment is deleted. Otherwise the course is considered as "failed".

Course Components

Type Title Time & Place Hours
practical/laboratory course Introduction to Mass Spectrometry-Based Proteomics
Permission from lecturers required for all students. Block course in the 1st quarter of the autumn semester
  • Tue 12:45-16:30 (HPZ E 35)
  • Wed 07:45-16:30 (HPZ E 35)
  • Thu 07:45-16:30 (HPZ E 35)
  • Fri 07:45-16:30 (HPZ E 35)
100 h semesterly

Offered In