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551-0352-00L 6 Credits BSC D-BIOL
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Introduction to Mass Spectrometry-Based Proteomics

Lecturers & Examiners: Prof. Dr. Paola Picotti, Dr. Ludovic Gillet
Number of participants limited to 12. The enrolment is done by the D-BIOL study administration. General safety regulations for all block courses: -Whenever possible the distance rules have to be respected -All students have to wear masks throughout the course. Please keep reserve masks ready. Surgical masks (IIR) or medical grade masks (FFP2) without a valve are permitted. Community masks (fabric masks) are not allowed. -The installation and activation of the Swiss Covid-App is highly encouraged -Any additional rules for individual courses have to be respected -Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible
VVZ CR n/a

Last Updated: 2026-02-05 15:53:53

Abstract

Protein Analysis by Mass SpectrometryThe following topics will be covered: basics of biological mass spectrometry, including instrumentation, data collection and data analysis; applications to protein identification and characterization; sample preparation methods; proteomics strategies; and quantitative analysis.

Objective

How to prepare a protein sample for MS analysis (trypsin digestion, C18 clean-up) Principles of data acquisition LC-MS (QTOF and/or Ion Trap instruments) Perform qualitative proteomic analysis (protein identification with Mascot and/or Sequest Softwares) Perform quantitative proteomic analysis (label-free and labeled analyses) Analyze/interpret the data to find up/down regulated proteins

General Information

Language
English
Levels
BSC
Frequency
Yearly recurring

Examination

Type
graded semester performance

Course Components

Type Title Time & Place Hours
practical/laboratory course Introduction to Mass Spectrometry-Based Proteomics
Permission from lecturers required for all students. Block course in the 2nd quarter of the spring semester
  • Tue 07:45-16:30 (HIT F 32)
  • Wed 07:45-16:30 (HIT F 32)
  • Thu 07:45-16:30 (HIT F 32)
  • Fri 07:45-16:30 (HIT F 32)
100 h semesterly

Offered In