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551-1003-00L 3 Credits BSC , MSC D-HEST , D-BIOL
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Methods of Biological Analysis

Methoden der Biologischen Analytik

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Last Updated: 2026-02-05 15:36:24

Abstract

529-1042-00Principles of the most important separation techniques and the interpretation of molecular spectra.551-1003-00The course will teach the basis and typical applications of methods for the analysis of nucleic acid sequences, mass spectrometric analysis of proteins and proteomes and advanced light and fluorescent imaging methods.

Objective

529-1042-00 Knowledge of the necessary basics and the possibilities of application of the relevant spectroscopical and separation methods in analytical chermistry. 551-1003-00 Knowledge of the theoretical basis of the methods for nucleic acid sequence analysis, mass spectrometry based protein and proteome analysis and advanced light and fluorescent imaging methods, and an understanding of the application of these principles in experimental biology.

Content

529-1042-00 Application oriented basics of instrumental analysis in organic chemistry and the empirical employment of the methods of structure elucidation (mass spectrometry, NMR-, IR-, UV/VIS spectroscopy). Basics and application of chromatographic and electrophoretic separation methods. Application of the knowledge by practising. 551-1003-00 The course will consist of lectures covering the theoretical and technical base of the respective analytical methods and of exercises where typical applications of the methods in modern experimental biology are discussed.

Resources

Lecture Notes

529-1042-00A comprehensive script is available in the HCI-Shop. A summary of the part "Spektroskopie" defines the relevant material for the exam.551-1003-00Materials supporting the lectures and exercises will be made available via Moodle.

Literature

529-1042-00 - Pretsch E., Bühlmann P., Badertscher M. Structure Determination of Organic Compounds, 5th revised and enlarged English edition, Springer-Verlag, Berlin 2009; - Pretsch E., Bühlmann P., Badertscher M., Spektroskopische Daten zur Strukturaufklärung organischer Verbindungen, fünfte Auflage, Springer-Verlag, Berlin 2010; - D.A. Skoog, J.J. Leary, Instrumentelle Analytik, Grundlagen, Geräte, Anwendungen, Springer, Berlin, 1996; - K. Cammann, Instrumentelle Analytische Chemie, Verfahren, Anwendungen, Qualitätssicherung, Spektrum Akademischer Verlag, Heidelberg, 2001; - R. Kellner, J.-M. Mermet, M. Otto, H.M. Widmer, Analytical Chemistry, Wiley-VCH Verlag, Weinheim, 1998; - K. Robards, P.R.Haddad, P.E. Jackson, Principles and practice of modern chromatographic methods, Academic Press, London, 1994;

Learning Materials (Links)

General Information

Language
German
Levels
BSC , MSC
Frequency
Yearly recurring

Examination

Type
session examination
Mode
written 150 minutes
Aids
Alle, ausser Computer und Telekommunikation. Taschenrechner erlaubt und empfohlen.
Sprache der Leistungskontrolle ist Deutsch oder Englisch

Course Components

Type Title Time & Place Hours
lecture with exercise Analytik
Die genauen Unterrichtszeiten von ONLINE - Veranstaltungen werden von den Dozierenden kommuniziert.
  • Mon 08:00-09:00 (ON LI NE)
  • Fri 10:00-12:00 (ON LI NE)
1.5 h weekly
lecture with exercise Methoden der Biologischen Analytik
Die genauen Unterrichtszeiten von ONLINE - Veranstaltungen werden von den Dozierenden kommuniziert.
  • Mon 08:00-09:00 (ON LI NE)
  • Fri 10:00-12:00 (ON LI NE)
1.5 h weekly

Offered In