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551-0363-00L 6 Credits BSC D-BIOL

Microbial Gene Technology

Mikrobielle Gentechnologie

Lecturers: Prof. em. Dr. Hans-Martin Fischer, Prof. Dr. Markus Künzler
Examiners: Prof. em. Dr. Hans-Martin Fischer
VVZ CR n/a

Last Updated: 2026-02-05 15:23:45

Abstract

Introduction to selected prokaryotic and eukaryotic model microorganisms and application of methods which are used as tools for answering questions in molecular biology.

Objective

Knowledge about the introduced microorganisms, about the methods which are introduced in the course and their application to answer general questions in molecular biology.

Content

A. Bakers yeast Saccharomyces cerevisiae as a model organism for investigating protein-N-glycosylation (PNG) in the endoplasmic reticulum - Introduction of mutations in a PNG gene by site-directed mutagenesis and in vivo recombination in yeast, functional analysis of the gene in yeast (Molecular genetics) - Complementation of mutations in a yeast PNG gene by an orthologous gene from another organism (Molecular and classical genetics) - Biochemical analysis of the N-glycans of a reporter protein in various yeast PNG mutants (Biochemistry) B. Transcriptome analysis of the soybean symbiont Bradyrhizobium japonicum with microarrays - Isolation of RNA and reverse transcription into cDNA - Fluorescent labeling of cDNA - DNA chip hybridization - Data analysis (bioinformatics)

Resources

Lecture Notes

The script and further information about this course will become available athttp://team.biol.ethz.ch/e-learn/551-0363-00L.To access the E-Learn portalhttp://team.biol.ethz.ch/e-learn:ETH students (D-BIOL, D-AGRL, D-CHAB, D-ERDW, D-UWIS) and UNIZH students:Username: D\"NETZ_username" or d.ethz.ch\"NETZ_username"Password: ETH-Email password (=NETZ password if not changed)

General Information

Language
English
Levels
BSC
Frequency
Yearly recurring

Examination

Type
graded semester performance
Es wird nur der Hauptexaminator aufgeführt. Weitere Examinatoren: Die oben aufgeführten Dozierenden.

Course Components

Type Title Time & Place Hours
lecture with exercise Mikrobielle Gentechnologie
Permission from lecturers required for all students. Blockkurs im 4. Viertel des Herbstsemesters max. 16 Teilnehmer
  • Tue 12:45-16:30 (HCI E 392)
  • Wed 07:45-16:30 (HCI E 392)
  • Thu 07:45-16:30 (HCI E 392)
  • Fri 07:45-16:30 (HCI E 392)
100 h semesterly

Offered In