Laboratory Practical in Molecular Animal Genetics and Inherited Diseases

Abstract

Technologies of molecular animal-, immuno- and biochemical genetics will be shown and applied to selected domestic and farm animals. The students will perform laboratory tests for genome analysis (identification of gene loci, gene mapping), gene expression (mRNA, proteins), diagnostics (analysis of hereditary diseases) and verification of animals and animal products (parentage control, forensics).

Objective

Get to know and apply the basic laboratory methods to systematically identify loci controlling important performance and disease traits and analysis of the molecular nature of variation at the loci. At the end of the course the students are able to understand the underlying mechanisms influencing genetic variations and to analyze gene variants in the laboratory. The students know the importance of the technologies for animal breeding, animal health and quality of animal products in Switzerland and internationally. The matter is illustrated on practical examples.

Content

- Introduction to the course (aims, program, written examination) - Porcine E.coli test. Determination of the mutation in FUT1 - Sequencing of DNA - Marker-/microsatellite analyses - Forensics - Cytogenetics - Cell cultures - Inheritance of coat colour - Gene expression and animal biotechnology - Blood groups and biochemical genetics for parentage verification Contact hours: 42 h Self-study (preparation for courses and examination): additionally

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