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551-0363-00L 6 Credits BSC D-BIOL
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Microbial Gene Technology

Mikrobielle Gentechnologie

Lecturers: Prof. em. Dr. Hans-Martin Fischer, Prof. Dr. Markus Künzler
Examiners: Prof. em. Dr. Hans-Martin Fischer
VVZ CR n/a

Last Updated: 2026-02-05 15:13:20

Abstract

Introduction to selected prokaryotic and eukaryotic model microorganisms and application of methods which are used as tools for answering questions in molecular biology.

Objective

Knowledge about the introduced microorganisms, about the methods which are introduced in the course and their application to answer general questions in molecular biology.

Content

A. Bakers yeast Saccharomyces cerevisiae as a model organism for investigating protein-N-glycosylation (PNG) in the endoplasmic reticulum - Isolation of temperature-sensitive mutations in a yeast PNG gene by low fidelity PCR and in vivo recombination; characterization of the obtained mutations by DNA sequencing - Complementation of mutations in yeast PNG genes by the human orthologs - Biochemical analysis of the N-glycans of a reporter protein in various yeast PNG mutants -Test of various PNG genes for genetic interaction according to a protocol of a high-throughput screen for synthetic lethal mutations in yeast B. Transcriptome analysis of the soybean symbiont Bradyrhizobium japonicum with microarrays - Isolation of RNA and reverse transcription into cDNA - Fluorescent labeling of cDNA - DNA chip hybridization - Data analysis (bioinformatics)

Resources

Lecture Notes

The script and further information about this course will become available athttp://team.biol.ethz.ch/e-learn/551-0363-00L.

General Information

Language
English
Levels
BSC
Frequency
Yearly recurring

Examination

Type
graded semester performance
Es wird nur der Hauptexaminator aufgeführt. Weitere Examinatoren: Die oben aufgeführten Dozierenden.

Course Components

Type Title Time & Place Hours
lecture with exercise Mikrobielle Gentechnologie
Permission from lecturers required for all students. Blockkurs im 4. Viertel des Herbstsemesters (29.11. - 21.12.07) max. 16 Teilnehmer
  • Tue 12:45-16:30 (HCI E 392)
  • Wed 07:45-16:30 (HCI E 392)
  • Thu 07:45-16:30 (HCI E 392)
  • Fri 07:45-16:30 (HCI E 392)
100 h semesterly

Offered In