VVZ API is not affiliated with ETH Zurich. Data might be outdated or incorrect. Please view the official ETHZ Vorlesungsverzeichnis for binding information.

551-0442-00L 6 Credits BSC D-BIOL
You're viewing possible stale or outdated data. Please check the latest semester for more up-to-date information.

Modern Concepts in Protein- and Proteom Analysis

Moderne Konzepte in der Protein - und Proteomanalyse

Lecturers: PD Dr. René Albert Brunisholz, Dr. Peter Hunziker
Examiners: PD Dr. René Albert Brunisholz
VVZ CR n/a

Last Updated: 2026-02-05 15:18:46

Abstract

Characterisation and identification of proteins and peptides using amino acid analysis, N-terminal sequencing, high performance liquid chromatography (HPLC) and mass spectrometry (MS).Overview on protein and proteom analytics and focussing on modern methods.

Objective

- Handling of very small amounts (< picomoles) of protein and peptide material- Understand the basic principles of mass spectrometry - Protein and peptide specific sample preparation for MALDI and ESI MS - Basic operations of various analytical apparatus - Learn to apply programs developed for MS; e.g. Biotools

Content

Potential types of experiments Experimental part 1: Characterisation of proteins: - Reductive alkylation of proteins - Disulfidemapping of proteins - Detect partially reduced protein specimen by HPLC and MS - Localize and identify artificially or naturally modified amino acids (chemical labels or post-translational modifications; e.g. phosphorylation sites) Analytical methods - Chemical and enzymatic fragmentation - Amino acid analysis - Edman sequencing - Capillary HPLC and MS coupling (LC-MALDI MS and LC-ESI MS) - Electrospray Ionisation (ESI) and Matrix Assisted Laser Desorption Ionisation (MALDI) MS - Tandem mass spectrometry (MS/MS)Experimental part 2: Identification of proteins from solution and after SDS PAGE: - In-gel digestion - Peptide mass fingerprinting (PMF) Analytical methods - Electrospray Ionisationa (ESI) and Matrix Assisted Laser Desorption Ionisation (MALDI) MS - Tandem MS (MS/MS) - Capillary HPLC and MS coupling (LC-MALDI MS and LC-ESI MS) - Protein identification by using Mascot and other search engines

General Information

Language
German
Levels
BSC
Frequency
Yearly recurring

Examination

Type
graded semester performance
Es wird nur der hauptverantwortliche Examinator aufgeführt. Weitere Examinatoren: Die oben aufgeführten Dozierenden.

Course Components

Type Title Time & Place Hours
lecture with exercise Modern Concepts in Protein- and Proteom Analysis
Blockkurs im 4. Viertel des Sommersemesters: 1.06.07 - 22.06.07 Beschränkt auf max 4 Teilnehmer Ort: Functional Genomics Center Zürich, Universitätskampus Irchel 8057 Zürich Winterthurerstrasse 190, Bau 32 Labors H22, H24 und H26
No time listed 100 h semesterly

Offered In