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551-0372-00L 6 Credits BSC D-BIOL
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Genome-wide RNA Interference, Imaging and Computational Image Analysis in Systems Biology

Lecturers & Examiners: Dr. Lucas Pelkmans
VVZ CR n/a

Last Updated: 2026-02-05 15:18:46

Abstract

A major goal of post-genomic research is to annotate functions to each gene of the human genome. Systematic siRNA screens, in which each gene is silenced with a specific siRNA, combined with automated imaging and computational image-analysis to quantify cellular phenotypes, now allow us to do this in human cells. We will apply it in this course to study a poorly characterized cellular system.

Objective

A major goal of post-genomic research is to annotate functions to each gene of the human genome. Systematic siRNA screens, in which each gene is silenced with a specific siRNA, combined with automated imaging and computational image-analysis to quantify cellular phenotypes, now allow us to do this in human cells. As such screens are large they must be automated, and light microscopy is currently the most powerful approach to study phenotypes of individual human cells in an automated manner. We will conduct such a screen during the course, in which we will analyze a poorly characterized cellular system. The data that are generated will thus be truly novel. While generation of datasets is relatively easy, it is much more challenging to analyze them in a proper way. To do this in an unbiased and quantitative manner for large amounts of cells, we will analyze each image with a set of computer algorithms. In the course, we will determine which algorithms are most appropriate for describing and distinguishing the phenotypes of the system that is studied. We will also use methods to classify and group similar phenotypes together, and to look at systems properties of the phenotypes at the level of individual cells within a large cell population.

General Information

Language
German
Levels
BSC
Frequency
Yearly recurring

Examination

Type
graded semester performance

Course Components

Type Title Time & Place Hours
lecture with exercise Genome-wide sRNA Interference
Blockkurs im 4. Viertel des Sommersemesters Ort: HPT B72 (beschränkt auf max. 15 Teilnehmende)
No time listed 100 h semesterly

Offered In