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Biological Chemistry: Directed Evolution of Proteins
Last Updated: 2026-02-05 15:02:28
Abstract
The class is a "block course" (with a very dense program), consisting of the practical course itself and an integrated series of seminar/lecture sessions. All technologies used for the experiments will be explained to the students in theory and in practice with the goal that they will be able to independently apply them in the last week of the course.
Content
We intend to carry out biological-chemical enzyme evolution experiments using molecular genetic mutation technologies and in vivo selection in recombinant bacterial strains. By working in parallel, the 12 student teams composed of 2 participants each should evolve a variety of different variants of a catalyst. Individual proteins will be purified and subsequently characterized using several different spectroscopic methods. The detailed chemical-physical analyses include determination of the enzymes' kinetic parameters and the integrity of the secondary structure. The results obtained from the individual evolution experiments will be assembled, compared and discussed at the end of the class in a final seminar. We expect that during this lab course we will not only generate novel enzymes, but also gain new insights into the reaction mechanism of the investigated catalysts.
Resources
Lecture Notes
A script will be distributed to the participants on the first day of the course.
General Information
- Language
- English
- Frequency
- Yearly recurring
Examination
- Type
- ungraded semester performance
Course Components
| Type | Title | Time & Place | Hours |
|---|---|---|---|
| practical/laboratory course |
Biological Chemistry: Directed Evolution of Proteins
Permission from lecturers required for all students.
Blockpraktikum in den Frühlingsferien // Lokal: HCI H2 / HCI H294 / HCI H296
Informationen zur Anmeldung:
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20 h weekly |
Offered In
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Praktikum oder Semesterarbeit (Achtung: Laut Master Reglement müssen für das Master Diplom gesamthaft entweder ein Praktikum und eine oder zwei Semesterarbeiten in den Kern- oder Wahlfachbereichen absolviert werden.)
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