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Abstract
Mammalian cells will be transfected and transduced for the production of biopharmaceuticals, for drug discovery as well as for the design of synthetic biology-inspired programmable gene circuits. A wide array of analytical techniques, lectures, and excursions to biotech companies will complement the practical part.
Objective
Independent planning and conducting of experiments with mammalian cells including all steps from culturing different cell lines to DNA transfection/transduction and expression analysis using a wide array of analytical methods.
Content
A practical course on characterization and cultivation of mammalian cells, DNA transfer by transfection, construction of synthetic gene networks, analysis of gene expression by enzymatic and immunological methods and fluorescent proteins, bioprocessing, mammalian cell-based assays for drug discovery and diagnostics. Excursions to Biotech/Pharma companies.
Resources
Lecture Notes
Will be distributed on first day of the practical course