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Abstract
The lecture introduces two methods for the structure determination ofbiological macromolecules and cellular components: X-ray crystallography and electron microscopy (EM).
Objective
To understand the basic concepts of protein crystallography and electron microscopy in theory and practice.
Content
The lecture introduces two methods for the structure determination of biological macromolecules and cellular components: X-ray crystallography and electron microscopy (EM). The lecture provides students with the main concepts of protein structure determination by X-ray crystallography (protein crystallization, crystal symmetry and diffraction, data collection, phasing methods, refinement). The second part of the lecture will deal with electron microscopy. The topics include Transmission EM, Scanning EM, sample preparation, data acquisition, 3D reconstruction, aberration, detectors.