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Abstract
The course covers all basic aspects of metabolome measurements, from sample sampling to mass spectrometry and data analysis. Participants work in groups and independently perform and interpret metabolomic experiments.
Objective
Performing and reporting a metabolomic experiment, understanding pro and cons of mass spectrometry based metabolomics. Knowledge of workflows and tools to assist experiment interpretation, and metabolite identification.
Content
Basics of metabolomics: workflows, sample preparation, targeted and untargeted mass spectrometry, instrumentation, separation techniques (GC, LC, CE), metabolite identification, data interpretation and integration, normalization, QCs, maintenance. Soft skills to be trained: project planning, presentation, reporting, independent working style, team work.