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636-0204-00L 2 Credits MSC D-BSSE

Lab Course: Microbial Biotechnology

Lecturers & Examiners: Dr. Tania Roberts
The lab course is open for MSc Biotechnology students only.
VVZ CR n/a

Last Updated: 2026-06-03 00:07:32

Abstract

Students will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels

Objective

Students will learn the foundations of monoseptic working practice and create and screen microbial libraries for identification of strains expressing different fluorescent protein (XFP) levels

Content

Block A: Handling and preparation and of microbial libraries D1: Introduction to microbiological cultures and monoseptic working techniques. D2: Plasmid-based expression systems and variation of XFP synthesis levels via site-directed RBS mutagenesis. Block B: Library screening D3: In vivo screening for XFP expression levels. D4: Analysis of XFP levels via SDS-PAGE analysis. RBS-sequencing. Block C: Hit recovery and validation D5: In silico analysis of RBS variants. D6: Cellular XFP content for selected variants at different culture conditions. Block D: Data analysis and presentation D7: Protein expression analysis. Q&A for reports and presentations. D8: Final presentations and wrap-up.

Resources

Lecture Notes

Material will be provided during the course.

Literature

(1) Reetz MT, Kahakeaw D, and Lohmer R. "Addressing the numbers problem in directed evolution." ChemBioChem 2008 (2) Jeschek M, Gerngross D, and Panke S. “Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort.” Nat. Commun. 2016 (3) Salis HM. “The ribosome binding site calculator.” Methods Enzymol. 2011 (4) Nienhaus G, Nienhaus K, and Wiedenmann J. "Structure–Function Relationships in Fluorescent Marker Proteins of the Green Fluorescent Protein Family." Fluorescent Proteins I. Springer Berlin Heidelberg, 2011 General introduction to microbiology: (5) Schlegel HG, and Zaborosch C. “General Microbiology.” Cambridge University Press 1993 (6) Pirt JS. “Principles of microbe and cell cultivation.” Blackwell Scientific Publications 1975

General Information

Language
English
Levels
MSC
Frequency
Yearly recurring

Examination

Type
ungraded semester performance

Registration & Places

Priority: Registration for the course unit is only possible for the primary target group

Course Components

Type Title Time & Place Hours
practical/laboratory course Lab Course: Microbial Biotechnology
The course takes place Mondays/Tuesdays on the following dates: 23-24 November | 30 November -1 December / 7-8 / 14-15 December
No time listed 64 h semesterly

Offered In