Toxicology and Nutrition Laboratory Course

Abstract

Introduction to the analysis of pesticide residues in food, learning to assess cytotoxicity of a chemical in cells, monitoring the stability of DNA by UV spectrophotometry and synthesizing an antioxidant.Introduction to the topics cardiometabolic risk and glycemic response, including dietary assessment, anthropometric measurements and determination of relevant biomarkers.

Objective

1) To gain practical insights into food monitoring by applying an LC-MS screening method and legal requirements for pesticides. 2) To learn details about mammalian cell cultures and practical training on the use of methods to measure cell viability. 3) To study the properties of a DNA duplex by melting the native structure while monitoring the transition with UV spectrophotometry. 4) To preform an organic chemistry reaction and recrystallization of an antioxidant and use UV spectrophotometry to assess the antioxidant capacity of structurally similar compounds. 5) To learn the concept of cardiometabolic risk and to measure the relevant biomarkers using point of care methods. 6) Application of a dietary assessment method and analysis of the protocols using dietary software. 7) To learn to assess and interpret anthropometric measurements, such as assessment of growth, fat-free mass and body fat 8) To get insights into the topic of glycemic response and to measure glycemic response to different meals.

Content

1) To gain practical insights into food monitoring by applying an LC-MS screening method and legal requirements for pesticides. 2) To learn details about mammalian cell cultures and practical training on the use of methods to measure cell viability. 3) To study the properties of a DNA duplex by melting the native structure while monitoring the transition with UV spectrophotometry. 4) To preform an organic chemistry reaction and recrystallization of an antioxidant and use UV spectrophotometry to assess the antioxidant capacity of structurally similar compounds. 5) To learn the concept of cardiometabolic risk and to measure the relevant biomarkers using point of care methods. 6) Application of a dietary assessment method and analysis of the protocols using dietary software. 7) To learn to assess and interpret anthropometric measurements, such as assessment of growth, fat-free mass and body fat 8) To get insights into the topic of glycemic response and to measure glycemic response to different meals.

Resources