Ecotoxicology - Laboratory

Abstract

This course enables students to become familiar with state-of-the-art concepts and methods of molecular ecotoxicology. We explore mechanisms of chemicals toxicity in fish cells and embryos, i.e. alternatives to conventional animal tests. The course is organized in theoretical and practical training components, including data evaluation/presentation. Students work both in class and in small groups.

Objective

Molecular methods are crucial for shedding light on mechanisms underlying biological structure and function under normal and stress conditions. The aim of this course it to demonstrate the power of these methods but also their limits and to enable students to appreciate them both in theoretical and practical terms.

Content

Training comprises designing and carrying out of chemical exposure experiments and assessment of disturbances in fish cells and embryos, such as impact on viability, regulation of genes, sub-lethal developmental effects and behavioral responses. Applied techniques include cell/embryo culture, microscopy, DNA/RNA isolation and gene expression, behavioral video analysis and statistics.

Resources