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227-0390-00L 4 Credits DR , MSC , NDS D-CHAB , D-MAVT , D-PHYS , D-ITET , D-HEST , D-BIOL
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Elements of Microscopy

Lecturers: Dr. Gábor Csúcs, Prof. Dr. Marco Stampanoni, Dr. Alla Sologubenko
Examiners: Prof. Dr. Marco Stampanoni, Dr. Alla Sologubenko
VVZ CR n/a

Last Updated: 2026-06-01 11:33:01

Abstract

The lecture reviews the basics of microscopy by discussing wave propagation, diffraction phenomena and aberrations. It gives the basics of light microscopy, introducing fluorescence, wide-field, confocal and multiphoton imaging. It further covers 3D electron microscopy and 3D X-ray tomographic micro and nanoimaging.

Objective

Solid introduction to the basics of microscopy, either with visible light, electrons or X-rays.

Content

It would be impossible to imagine any scientific activities without the help of microscopy. Nowadays, scientists can count on very powerful instruments that allow investigating sample down to the atomic level. The lecture includes a general introduction to the principles of microscopy, from wave physics to image formation. It provides the physical and engineering basics to understand visible light, electron and X-ray microscopy. During selected exercises in the lab, several sophisticated instrument will be explained and their capabilities demonstrated.

Resources

Literature

Available Online.

General Information

Language
English
Levels
DR , MSC , NDS
Frequency
Yearly recurring

Examination

Type
session examination
Mode
oral 30 minutes

Course Components

Type Title Time & Place Hours
lecture with exercise Elements of Microscopy
  • Mon 08:50-11:30 (HIL D 10.2)
3 h weekly

Offered In