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327-2125-00L 2 Credits DR , MSC D-HEST , D-MATL
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Microscopy Training SEM I - Introduction to SEM

Limited number of participants. Master students will have priority over PhD students. PhD students may still enroll, but will be asked for a fee.
VVZ CR n/a

Last Updated: 2026-06-01 11:33:18

Abstract

The introductory course on Scanning Electron Microscopy (SEM) emphasizes hands-on learning. Using 2 SEM instruments, students have the opportunity to study their own samples, or standard test samples, as well as solving exercises provided by ScopeM scientists.

Objective

- Set-up, align and operate a SEM successfully and safely. - Accomplish imaging tasks successfully and optimize microscope performances. - Master the operation of a low-vacuum and field-emission SEM and EDX instrument. - Perform sample preparation with corresponding techniques and equipment for imaging and analysis - Acquire techniques in obtaining secondary electron and backscatter electron micrographs - Perform EDX qualitative and semi-quantitative analysis

Content

During the course, students learn through lectures, demonstrations, and hands-on sessions how to setup and operate SEM instruments, including low-vacuum and low-voltage applications. This course gives basic skills for students new to SEM. At the end of the course, students with no prior experience are able to align a SEM, to obtain secondary electron (SE) and backscatter electron (BSE) micrographs and to perform energy dispersive X-ray spectroscopy (EDX) qualitative and semi-quantitative analysis. The procedures to better utilize SEM to solve practical problems and to optimize SEM analysis for a wide range of materials will be emphasized. - Discussion of students' sample/interest - Introduction and discussion on Electron Microscopy and instrumentation - Lectures on electron sources, electron lenses and probe formation - Lectures on beam/specimen interaction, image formation, image contrast and imaging modes. - Lectures on sample preparation techniques for EM - Brief description and demonstration of the SEM microscope - Practice on beam/specimen interaction, image formation, image contrast (and image processing) - Student participation on sample preparation techniques - Scanning Electron Microscopy lab exercises: setup and operate the instrument under various imaging modalities - Lecture and demonstrations on X-ray micro-analysis (theory and detection), qualitative and semi-quantitative EDX and point analysis, linescans and spectral mapping - Practice on real-world samples and report results

Resources

Literature

- Detailed course manual - Williams, Carter: Transmission Electron Microscopy, Plenum Press, 1996 - Hawkes, Valdre: Biophysical Electron Microscopy, Academic Press, 1990 - Egerton: Physical Principles of Electron Microscopy: an introduction to TEM, SEM and AEM, Springer Verlag, 2007

General Information

Language
English
Levels
DR , MSC
Frequency
Semesterly recurring

Examination

Type
ungraded semester performance
Oral presentation and discussion about the SEM images and results obtained during the course.

Registration & Places

Limited places (Special selection)
Signup End
03.03.2025

Course Components

Type Title Time & Place Hours
practical/laboratory course Microscopy Training SEM I - Introduction to SEM
This block course will take place during 5 full days (9am-5pm) on March 17-21, 2025. Lectures will be held in the seminar room, practical exercises in rooms of ScopeM. There will be no repetition of this course, but it's offered every semester.
  • 17.03 Date 07:45-13:30 (HPZ E 35)
  • 18.03 Date 07:45-13:30 (HPZ E 35)
  • 19.03 Date 07:45-13:30 (HPZ E 35)
  • 21.03 Date 11:45-15:30 (HPZ E 35)
35 h semesterly

Offered In