Gene Technology (Crash Course)

Abstract

The course enables the student to understand and and apply the general concepts of gene technology, including recombinant DNA technology and its application in genomics, transcriptomics and proteomics. Protein cloning, expression and modifications and bimolecular interactions will be discussed. The concept of display technology and its applications in the field of drug discovery will be presented.

Objective

The students remember and understand: 1. The tools of recombinant DNA technology 2. Next generation sequencing approaches and their relevance for -omics projects 3. Protein cloning, expression, modification/labelling and oligomerization 4. Thermodynamic and kinetic affinity constants in bimolecular reactions 5. Basic structure of the antibody molecule 6. Concepts of antibody phage technology and antibody engineering 7. Construction of antibody-, peptide- or small molecule libraries and affintiy-based selection methodologies

Content

I) Genomics: recombinant DNA technology methods to sequence genomes application to human biology Transcriptomics / Proteomics II) Proteins: protein cloning and expression homo- and heterodimerization chemical modifications and radioactive labelling detection of bimolecular interactions affinity constant and experimental measurement kinetic association and dissociation constants III) Display technology: the antibody molecule, CDRs, basics of antibody engineering antibody phage display and selection methodologies construction of antibody libraries other display technologies (peptide display, DNA-encoded chemical libraries)

Resources