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Microscopy Training Cryogenic Electron Microscopy
Last Updated: 2026-02-05 16:39:09
Abstract
The introductory course on cryogenic electron microscopy (cryoEM) provides theoretical and hands-on learning for new operators, utilizing lectures, demonstrations and hands-on sessions.
Objective
- Overview of cryoEM theory, instrumentation, operation and applications - Prepare cryoEM sample (vitrification using Vitrobot) - Set-up, align and operate a cryoTEM successfully and safely - Set up automated data collection - Basic processing steps to analyze/interpret the data e.g., reconstruction 3D volumes
Content
This course introduces and gives an overview of cryoEM and its applications. At the end of the course, students will be familiar with how to prepare vitrified probe and how to use a cryoTEM to collect and analyze data for exemplary techniques: - Introduction and discussion on cryoEM and instrumentation - Lectures on cryoEM theory - Lectures on cryoEM applications - Practicals/demonstration on vitrification, grid preparation - Practicals/demonstration on data collection - Lecture and practicals/demonstration on reconstruction of 3D volumes from 2D cryoEM projections/images
Resources
Literature
- Course slides - EM-University: ( https://em-learning.com/ ) - Book: CryoEM Methods and Protocols edited by T Gonen, B B Nannenga - Book: Single-particle Cryo-eM of Biological Macromolecules edited by R M Glaeser, E Nogales, W Chiu
General Information
- Language
- English
- Levels
- DR , MSC
- Frequency
- Semesterly recurring
Examination
- Type
- ungraded semester performance
Registration & Places
- Signup End
- 10.06.2024
Course Components
| Type | Title | Time & Place | Hours |
|---|---|---|---|
| practical/laboratory course |
Microscopy Training Cryogenic Electron Microscopy
Does not take place this semester.
|
No time listed | 21 h semesterly |
Offered In
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Biomedical Engineering Master (Only courses offered under "GESS Science in Perspective" count in this category. See "Offered in" tab in course view. For more information, please refer to )
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Recommended Elective Courses (These courses are particularly recommended for the Bioimaging track. Please consult your track adviser if you wish to select other subjects.)
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Elective Courses (The students are free to choose individually from the entire course offer of ETH Zürich on the Master level. Please consult the study administration in case of questions.)
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Doctorate Biology (More Information at: )
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Doctorate Materials Science (Further information at: )
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