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636-0121-00L 4 Credits DR , MSC D-BSSE
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Single Cell Technologies

Lecturers & Examiners: Prof. Dr. Barbara Treutlein
VVZ CR n/a

Last Updated: 2026-02-05 16:37:07

Abstract

Single-cell sequencing and imaging technologies are being applied to primary human organs and to engineered cells and tissues to understand cell states that emerge in these systems at unparalleled resolution. These technologies require sophisticated experimental and computational methods, which we will discuss in practical detail in this course.

Objective

To understand the history and current state of the art of single-cell sequencing and imaging methods, and to learn data analytical techniques to extract biological insight from the high-information content data.

Content

This course will include lecture sessions and paper discussion seminars, along with wet lab single-cell genomic experiments, followed by computational data analysis sessions based in R. In the lecture, I will cover the molecular biology and technical aspects underlying popular single-cell sequencing methods, as well as methods to spatially localize cell states within tissues or other multi-cellular systems. We will also cover the experimental aspects of light sheet microscopy and other microscopy methods as a tool to analyze cellular dynamics within complex tissues. We will read recent, seminal manuscripts in the single-cell genomics field and discuss the papers in detail as a group. Seminar topics will include: Single-cell RNA/DNA/Epigenome sequencing, Lineage tracing, Perturbation screens, Trajectory reconstruction, Light sheet microscopy In the drylab part of this course, we will go through each step of sequencing read processing and quality control analysis, followed by sessions on data exploration (cell composition, marker gene detection, trajectory reconstruction, differential gene expression analysis, RNA velocity analysis, condition comparison etc.)

General Information

Language
English
Levels
DR , MSC
Frequency
Yearly recurring

Examination

Type
end-of-semester examination
Mode
written 90 minutes
Aids
None
A presentation of the drylab analysis results will be graded as pass/fail and is required to pass the course.

Course Components

Type Title Time & Place Hours
lecture with exercise Single Cell Technologies
This lecture will be recorded.
  • Fri 14:15-17:00 (BSS E 27)
3 h weekly

Offered In