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327-2144-00L 1 Credits MSC D-MATL
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Microscopy Training Cryogenic Electron Microscopy

Lecturers & Examiners: Dr. Eszter Barthazy Meier, Dr. Peng Zeng, Stephan Handschin, Dr. Miriam Susanna Lucas-Droste, Miroslav Peterek, Dr. Bilal Qureshi
Registration form:
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Last Updated: 2026-02-05 16:16:26

Abstract

The introductory course on cryogenic electron microscopy (cryoEM) provides theoretical and hands-on learning for new operators, utilizing lectures, demonstrations and hands-on sessions.

Objective

- Overview of cryoEM theory, instrumentation, operation and applications - Prepare cryoEM sample (vitrification using Vitrobot) - Set-up, align and operate a cryoTEM successfully and safely - Set up automated data collection - Basic processing steps to analyze/interpret the data e.g., reconstruction 3D volumes

Content

This course introduces and gives an overview of cryoEM and its applications. At the end of the course, students will be familiar with how to prepare vitrified probe and how to use a cryoTEM to collect and analyze data for exemplary techniques: - Introduction and discussion on cryoEM and instrumentation - Lectures on cryoEM theory - Lectures on cryoEM applications - Practicals/demonstration on vitrification, grid preparation - Practicals/demonstration on data collection - Lecture and practicals/demonstration on reconstruction of 3D volumes from 2D cryoEM projections/images

Resources

Literature

- Course slides - EM-University: ( https://em-learning.com/ ) - Book: CryoEM Methods and Protocols edited by T Gonen, B B Nannenga - Book: Single-particle Cryo-eM of Biological Macromolecules edited by R M Glaeser, E Nogales, W Chiu

General Information

Language
English
Levels
MSC
Frequency
Semesterly recurring

Examination

Type
ungraded semester performance
Student presentation and discussion

Registration & Places

Limited places (Special selection)
Signup End
30.10.2023

Course Components

Type Title Time & Place Hours
practical/laboratory course Microscopy Training Cryogenic Electron Microscopy
This 3-day block course takes place on November 13-15, 2023 in the seminar room and rooms of ScopeM.
  • 13.11 Date 07:45-13:30 (HIT F 11.1)
  • 14.11 Date 07:45-13:30 (HIT F 11.1)
  • 15.11 Date 07:45-17:30 (HIT F 11.1)
21 h semesterly

Offered In