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Introduction to Flow Cytometry
Last Updated: 2026-02-05 16:23:31
Abstract
This course provides a broad introduction to flow cytometry. We will cover the working principles of cytometry instrumentation, and essential aspects of experimental design and data analysis. Fluorescence-based cytometry (conventional and full spectrum) as well as mass cytometry will be covered.The format is a lecture course enriched by a visit to the ETH Flow Cytometry Core Facility.
Objective
The objective of this course is to provide participants with a strong understanding of the basic principles underlying flow cytometry, equipping them to be self-sufficient for the systematic design, execution and analysis of cytometry experiments.
Content
The course is centered on explaining the working principles of cytometry instrumentation (conventional, full spectrum and mass cytometry) and associated data analysis. In detail, we will cover the following topics: - Components of Flow Cytometry Instrumentation - Signal processing - Fluorochromes, compensation, and spectral unmixing - Visualization, gating and analysis of data - Fluorescent panel design - Cytometry assays: immunophenotyping, cell signaling, etc. - Cell sorting - Mass cytometry - Computational data analysis techniques
Resources
Lecture Notes
Updated handouts will be provided during the class.
Literature
The lecture will reference key literature on cytometry instrumentation, experimental design, immunophenotyping and other applications. For general reading, we recommend the following: - Maciorowski et al, Basic Multicolor Flow Cytomety, Current Protocols in Immunology 2017: https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpim.26 - Cossarizza et al, Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), European Journal of Immunology 2021: https://onlinelibrary.wiley.com/doi/10.1002/eji.202170126 - Liechti et al, An updated guide for the perplexed: cytometry in the high-dimensional era, Nature Immunology 2021: https://www.nature.com/articles/s41590-021-01006-z
General Information
- Language
- English
- Levels
- DR , MSC
- Frequency
- Yearly recurring
Examination
- Type
- session examination
- Mode
- written 60 minutes
- Aids
- None
Registration & Places
- Max Places
- 40
Course Components
| Type | Title | Time & Place | Hours |
|---|---|---|---|
| lecture | Introduction to Flow Cytometry |
|
1 h weekly |
Offered In
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Doctorate Biology (More Information at: )