Gentechnologie

Abstract

The aim of the lecture course is to provide a solid overview of the science and issues in gene technology and genome science. The topics covered include antibody phage technology, protein modification technology, genome projects, genome sequencing, transcriptomics, proteomics and SNP technology. The course is suited for advanced undergraduate and early graduate students in pharmaceutical sciences or related fields. A basic understanding of molecular biology and biochemistry is required.

Objective

The course will provide a solid overview of the science and issues in gene technology and genome science.

Content

1. Antibody phage technology The antibody molecule V genes, CDRs, basics of antibody engineering Principles of phage display Phagemid and phage vectors Antibody libraries Phage display selection methodologies Other phage libraries (peptides, globular proteins, enzymes) Alternative screening/selection methodologies Intrabodies 2. Proteins: chemical modification and detection of biomolecular interactions Homo- and hetero-dimerization of proteins Chemical modifications of proteins Radioactive labeling of proteins Kinetic association and dissociation constants Affinity constant: definition and its experimental measurement 3. Genome Projects: organization and objectives Core aims of genome science Mapping genomes The Human Genome Project Animal Genome Projects Genome Projects 4. Genome sequencing and annotation Automated DNA sequencing Genome sequencing Genome annotation Functional annotation and gene family clustering 5. Gene expression and the transcriptome Parallel analysis of gene expression: microarrays SAGE Single-gene analyses Properties of transcriptomes 6. Proteomics and functional genomics Functional Proteomics Functional Genomics 7. SNPs and variation The nature of SNPs Applications of SNP technology SNP discovery

Resources