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636-0207-00L 2 Credits MSC D-BSSE
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Lab Course: Cellular Engineering Stem Cells

Lecturers & Examiners: Prof. Dr. Timm Schroeder
The lab course is open for MSc Biotechnology students only.
VVZ CR n/a

Last Updated: 2026-02-05 16:07:58

Abstract

Mammalian stem cells of different organs are purified, cultured, differentiated, analyzed and manipulated. Plasmids and viral vectors will be cloned, produced and transfected / transduced to manipulate stem cells. Computational and analytical molecular biology methods, FACS and imaging and lectures complement the program.

Objective

Independent planning and conducting of experiments with mammalian stem cells including all steps from culturing different cell lines to DNA transfection / transduction and expression analysis by different analytical methods. Documenting and writing a report on conducted experiments and results.

Content

Practical course on purification of primary mammalian stem cells, culture of primary stem cells and stem cell lines, characterization, manipulation and differentiation of stem cells. Construction of plasmids or viral vectors for gene expression, DNA transfer by transfection and transduction, analysis of gene expression by fluorescent proteins, PCR, fluorescence-activated cell sorting (FACS), imaging. Documentation of experiments in a laboratory journal, writing of a report on the experiments and results.

General Information

Language
English
Levels
MSC
Frequency
Yearly recurring

Examination

Type
ungraded semester performance
Written report on experiments and results will be graded as pass/fail and is required to pass the course.

Course Components

Type Title Time & Place Hours
practical/laboratory course Lab Course: Cellular Engineering Stem Cells
This lab course will take place after the end-of-semester-exam-period of spring semester and the Cellular Engineering Mammalian Cells lab course: Monday, July 4 - Friday, July 15, 10 full days
No time listed 80 h semesterly

Offered In