Proteome Analyses

Objective

In the postgenomic era new scientific approaches have emerged including the identification of proteins, their regulation, posttranslational modifications and interactions. These approaches are summarized under the term Proteomics. The course comprises a theoretical and a practical introduction to mass spectrometry, the key technology for protein analyses, and is designed to enable the participant to use an ion trap mass spectrometer for protein identification purposes. Every participant is invited to bring a protein sample of interest (please contact [email protected] for details) that will be analyzed independently by the participant at the end of the course. An accompanying lecture series will present the most recent developments in the field of proteomics and give details about "differential display" methods (such as ICAT, 2D-electrophoresis) for the identification of differentially expressed proteins in biological samples.

Content

In the postgenomic era new scientific approaches have emerged including the identification of proteins, their regulation, posttranslational modifications and interactions. These approaches are summarized under the term Proteomics. The course comprises a theoretical and a practical introduction to mass spectrometry, the key technology for protein analyses, and is designed to enable the participant to use an ion trap mass spectrometer for protein identification purposes. Every participant is invited to bring a protein sample of interest (please contact [email protected] for details) that will be analyzed independently by the participant at the end of the course. An accompanying lecture series will present the most recent developments in the field of proteomics and give details about "differential display" methods (such as ICAT, 2D-electrophoresis) for the identification of differentially expressed proteins in biological samples.

Resources