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551-1700-00L 2 Credits DR , MSC D-BIOL

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Introduction to Flow Cytometry

Lecturers: Dr. Jan Kisielow, Dr. Luigi Tortola

Examiners: Dr. Jan Kisielow

Number of participants limited to 24.

VVZ CR n/a

Last Updated: 2026-02-05 15:53:50

Abstract

The lecture provides an introduction to flow cytometry. We will cover the technology basics, experimental design, data acquisition and analysis of flow and mass cytometry. In addition, various research applications will be discussed. The format is a lecture course enriched by a visit to the ETH Flow Cytometry Core Facility and practical demonstration of the use of analysis and sorting instruments.

Objective

The goal of this course is to provide the basic knowledge of flow and mass cytometry required for planning and execution of cytometric experiments.

Content

The lecture course aims at teaching principles of flow cytometry. The emphasis is on theoretical principles (signal detection, fluorochromes, signal spill-over and compensation) as well as practical aspects of experimental design and performance (sample preparation, controls, data acquisition and analysis). List of topics: - Principles of Flow Cytometry - Signal processing - Compensation and Controls - Data analysis, gating and presentation - Panel design - Sorting - Mass cytometry - High-dimensional data analysis - Practical demonstration (hardware and software) Modern flow cytometric techniques for immunophenotyping, analysis of proliferation, cell cycle, apoptosis and cell signalling will be introduced.

Resources

Lecture Notes

Updated handouts will be provided during the class.

Literature

Current literature references on immunophenotyping, analysis of proliferation, cell cycle, apoptosis and cell signalling will be discussed during the lectures.

General Information

Language: English
Levels: DR , MSC
Frequency: Yearly recurring

Examination

Type: session examination
Mode: written 60 minutes
Aids: None

Only the main examiner is listed above.

Registration & Places

Max Places: 24

Course Components

Type	Title	Time & Place	Hours
lecture	Introduction to Flow Cytometry	Tue 08:45-09:30 (HPL D 32)	1 h weekly