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551-1554-00L 6 Credits BSC D-BIOL

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Multigene Expression in Mammalian Cells

Lecturers & Examiners: Prof. em. Dr. Gebhard Schertler, Dr. Philipp Berger

Number of participants limited to 5. The enrolment is done by the D-BIOL study administration.

VVZ CR n/a

Last Updated: 2026-02-05 15:41:03

Abstract

Genetic engineering of mammalian cells with multiple expression cassettes is an essential need in contemporary cell biology. It is useful for protein expression for structural studies, the reprogramming of somatic cells, or for the expression of several fluorescently-tagged sensors. In this course, we use MultiLabel (Kriz et al., Nat. Commun., 2010) to create multigene expression plasmids.

Objective

Students will learn to design and clone multigene expression constructs for mammalian cells. The functionality of the constructs will be tested by immunofluorescence microscopy or Western blotting.

Content

We will clone fluorescently-tagged markers for subcellular compartments, assemble them to a multigene expression construct and transfect them into mammalian cells. These markers of subcellular compartments will be used to study the trafficking of activated receptors (e.g. serotonin receptor). Pictures will be taken on our microscopes and then we will quantify colocalization.

Resources

Lecture Notes

none

General Information

Language: English
Levels: BSC
Frequency: Yearly recurring

Examination

Type: graded semester performance

Assessment during the course, Presentation, Written report (1/3 each), German/English

Course Components

Type	Title	Time & Place	Hours
lecture with exercise	Multigene Expression in Mammalian Cells Permission from lecturers required for all students. Block course in the 2nd quarter of the spring semester Place: Paul Scherrer Institut	No time listed	100 h semesterly

Offered In

Biology Bachelor
- 3. Year, 6. Semester
  - Block Courses (Registration for Block courses is mandatory. Please register under . Registration period from 16.12.2019 - 06.01.2020 Please note the ETH admission criteria for the admission of ETH students to ETH block courses on the block course registration website under "allocation".)
    - Block Courses in 2nd Quarter of the Semester (From 12.3.2020 to 2.4.2020)