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551-1554-00L 6 Credits BSC D-BIOL
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Multigene Expression in Mammalian Cells

Lecturers & Examiners: Dr. Philipp Berger
Does not take place this semester. Number of participants limited to 5. The enrolment is done by the D-BIOL study administration.
VVZ CR n/a

Last Updated: 2026-02-05 16:08:10

Abstract

Genetic engineering of mammalian cells with multiple expression cassettes is an essential need in contemporary cell biology. It is useful for protein expression for structural studies, the reprogramming of somatic cells, or for the expression of several fluorescently-tagged sensors. In this course, we use MultiLabel (Kriz et al., Nat. Commun., 2010) to create multigene expression plasmids.

Objective

Students will learn to design and clone multigene expression constructs for mammalian cells. The functionality of the constructs will be tested by immunofluorescence microscopy or Western blotting.

Content

We will clone fluorescently-tagged markers for subcellular compartments, assemble them to a multigene expression construct and transfect them into mammalian cells. These markers of subcellular compartments will be used to study the trafficking of activated receptors (e.g. serotonin receptor). Pictures will be taken on our microscopes and then we will quantify colocalization.

Resources

Lecture Notes

none

General Information

Language
English
Levels
BSC
Frequency
Yearly recurring

Examination

Type
graded semester performance
Assessment during the course, Presentation, Written report (1/3 each), German/EnglishStudents are obliged to be present throughout the block course.Cancellation: If you have to deregister from a course that has been assigned to you (just emergency reasons), please notify in written the course coordinator at least four weeks before the course starts, for courses in the 1st quarter, a cancellation period of one week applies. The study secretariat D-BIOL must also be informed (email CC [email protected]) so that the enrollment is deleted. Otherwise the course is considered as "failed".

Course Components

Type Title Time & Place Hours
practical/laboratory course Multigene Expression in Mammalian Cells
Does not take place this semester. Permission from lecturers required for all students. Block course in the 2nd quarter of the spring semester Place: Paul Scherrer Institut
No time listed 100 h semesterly

Offered In