VVZ API is not affiliated with ETH Zurich. Data might be outdated or incorrect. Please view the official ETHZ Vorlesungsverzeichnis for binding information.

551-1301-00L 20 Credits

Practical in Biochemistry and Molecular Biology

Biochemisches-molekularbiologisches Praktikum

Lecturers: PD Dr. René Albert Brunisholz, Dr. Ernesto Di Iorio, Prof. em. Dr. Timothy J. Richmond, Prof. em. Dr. Gerhard Wider, Prof. em. Dr. Rudolf Glockshuber, Dr. Imre Berger, Dr. Klaus Maskos, Prof. Dr. Eilika Weber-Ban, Prof. Dr. Frédéric Allain, Prof. Dr. Kaspar Locher, Dr. Horst Hemmo Meyer, Dr. Chrysoula Leontiou, Dr. Christiane Berger-Schaffitzel
Examiners: PD Dr. René Albert Brunisholz, Dr. Ernesto Di Iorio, Prof. em. Dr. Timothy J. Richmond, Prof. em. Dr. Gerhard Wider, Prof. em. Dr. Rudolf Glockshuber, Dr. Imre Berger, Dr. Klaus Maskos, Prof. Dr. Eilika Weber-Ban, Prof. Dr. Frédéric Allain, Prof. Dr. Kaspar Locher, Dr. Horst Hemmo Meyer, Dr. Chrysoula Leontiou
Beginn des Praktikums: 27.10.2005, 08.00 Uhr, Raum HPK C 1.1/9.
VVZ CR n/a

Last Updated: 2026-02-05 14:59:33

Abstract

Experimental methods for the purification, analysis and characterization of proteins and nucleic acids. Work on small research projects.

Objective

An introduction to experimental work on proteins and nucleic acids. Work on research projects.

Content

Block 1: Spectroscopical methods for the study of biological macromolecules [nuclear magnetic resonance (NMR), Electron Spin Resonance (ESR), fluorescence, and circular dichroism (CD) spectroscopy]. Block 2: Enzymatic and chemical fragmentation of proteins, SDS-polyacrylamide gel electrophoresis, electroblotting, amino-terminal sequencing of an unknown peptide using manual Edman degradation, high pressure liquid chromatography (HPLC), sequence analysis with the aid of data-bases, UV spectroscopy of proteins, quantitative determination of disulfide bonds, determination of the activation energy of non-catalyzed and and catalyzed reactions using starch degradation as an example. Block 3: Work on two small research projects. Block 4: Protein-DNA interactions and X-ray cristallography. Plasmid isolation, purification of DNA fragments, Analysis of the secondary structure of DNA and of transcription factors with DNA (gel retardation assay). overexpression of proteins in E.coli and enrichment via anion-exchanger, refolding of a multimeric protein complex from denatured components, preparation and analysis of a protein-DNA complex. X-ray crystallography of macromolecules, an overview: instrumentation, data analysis, computer-graphics.

Resources

Lecture Notes

Handouts will be distributed in the lab whenever needed.

Literature

None

General Information

Language
German
Frequency
Yearly recurring

Examination

Type
ungraded semester performance

Course Components

Type Title Time & Place Hours
practical/laboratory course Biochemisches-molekularbiologisches Praktikum
und nach Vereinbarung
  • Thu 07:45-16:30 (HPK C 1.1)
  • Thu 07:45-16:30 (HPK C 9)
  • Fri 07:45-16:30 (HPK C 1.1)
  • Fri 07:45-16:30 (HPK C 9)
  • 27.01 Date 07:45-09:30 (HPK H 10)
  • 10.02 Date 14:45-16:30 (HPK H 10)
20 h weekly

Offered In