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551-1275-02L 10 Credits

Microbial Gene Technology

Praktikum I in Biotechnologie: Teil Gentechnologie

Lecturers & Examiners: Prof. em. Dr. Hans-Martin Fischer, PD Dr. Pauli Kallio, Dr. Alexander Frey
VVZ CR n/a

Last Updated: 2026-02-05 14:59:33

Abstract

1A) Saccharomyces cerevisiae: a model organism to study human disorders 1B) Experimental strategies for site-directed mutagenesis in Salmonella typhimurium 1C) Affinity purification of a transcriptional regulator and demonstration of its DNA binding activity 2A) PCR amplification of genes from different microorganisms 2B) Site-directed mutagenesis of the hemoglobin gene from Vitreoscilla (vhb) 2C)

Objective

Application of gene technological methods to microbial systems.

Content

1A) Saccharomyces cerevisiae: a model organism to study human disorders 1B) Experimental strategies for site-directed mutagenesis in Salmonella typhimurium 1C) Affinity purification of a transcriptional regulator and demonstration of its DNA binding activity 2A) PCR amplification of genes from different microorganisms 2B) Site-directed mutagenesis of the hemoglobin gene from Vitreoscilla (vhb) 2C) Sequencing of the resulting mutant variants 2D) Analysis of clinical Pseudomonas isolates

Resources

Lecture Notes

Will be provided

Literature

None

General Information

Language
German
Frequency
Yearly recurring

Examination

Type
ungraded semester performance

Course Components

Type Title Time & Place Hours
practical/laboratory course Praktikum Mikrobielle Gentechnologie
3 Wochen Block (13.02.-03.03.2006) anschliessend ans Wintersemester Lokal: HCI E394, HCI 396
No time listed 9 h weekly

Offered In